mouse cd25 treg cell positive selection kit  (STEMCELL Technologies Inc)

Journal: Apoptosis

Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

doi: 10.1007/s10495-020-01629-x

Figure Lengend Snippet: Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of Treg cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots

Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

Techniques: Selection, Staining

Journal: Apoptosis

Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

doi: 10.1007/s10495-020-01629-x

Figure Lengend Snippet: a Treg cells express higher level of apoptosis-related proteins than CD4 + T cells. Analysis of 21 apoptosis-related protein expression in the cell lysates of untreated, sorted pTreg cells and CD4 + splenocytes. Bars show the average OD values of each analyte from at least three independent experiments (n = 6). b Effect of DX treatment on the expression of 21 apoptosis-related molecules in T cell subpopulations. Protein arrays were used to detect the in vitro DX treatment (4 h) induced changes of apoptosis-related molecules in cell lysates of sorted CD4 + T cells and pTreg cells. Bars show the DX-induced relative changes (%) of the average OD values of each analyte compared to untreated controls from at least three independent experiments (n = 6). Relative changes were calculated with the following formula: [(OD DX – OD Ctrl )/OD Ctrl ] × 100%

Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

Techniques: Expressing, In Vitro

Journal: Apoptosis

Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

doi: 10.1007/s10495-020-01629-x

Figure Lengend Snippet: Effect of DX on basal Ca 2+ levels in tTreg and pTreg cells. Short-term (30 min) DX treatment induced elevation of basal cytosolic free Ca 2+ levels in thymic Treg cells. In both organs, the baseline Ca 2+ levels were higher in Treg cells than in the corresponding CD4 + T cells. The columns show the Fluo 3-AM fluorescence intensities (MFI) of Tregs minus MFI of CD4 + T cells. Data represent mean ± SEM (n = 4). Significant change is indicated with asterisk ( p < 0.05)

Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

Techniques: Fluorescence

Journal: Apoptosis

Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

doi: 10.1007/s10495-020-01629-x

Figure Lengend Snippet: Calcium response is low in thymic and splenic Treg cells. Effect of TCR (anti-CD3) stimulation in thymic CD4 SP and tTreg cells was measured in the presence or absence of DX ( a ). Calcium influx kinetics in splenic CD4 + and Treg cells upon specific TCR-mediated activation (anti-CD3) in the presence or absence of DX ( b ). Data were calculated as the relative ratio of calcium values of Fluo 3-AM fluorescence intensities (median). Columns show the relative ratio of peak to baseline calcium flux measured as Fluo 3-AM fluorescence intensities (MFI) ( c ). The graphs show the mean ± SEM of at least three independent experiments (n = 9). Significant changes are indicated with asterisk (* p < 0.05, ***p < 0.001)

Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

Techniques: Activation Assay, Fluorescence

Journal: PLoS ONE

Article Title: Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio

doi: 10.1371/journal.pone.0189617

Figure Lengend Snippet: (A)The ratio of Treg in CD4+ T cells. (B) Representative diagram showing in comparison with the control group, IL-35 markedly enhanced the proliferation of Tregs (50.7% in IL-35 group vs. 9.5% in control group on day 5, P<0.01) under these conditions with time. Results are expressed as means ± SEM, from two experiments (n = 3 times/group/experiment). Unpaired t-tests were performed for comparisons between control- and IL-35- groups on corresponding days. *, ** and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: CD4+, CD4+CD25+, IL-17A+, and CD4+CD25- T cells were sorted by using the Stemcell magnetic sorter (cat: 18000), the human/mouse CD4 T cell negative selection kit (cat: 19852), the human/mouse Treg positive selection kit (cat: 18782), following the manufacturer’s instructions.

Techniques: Comparison, Control

Journal: PLoS ONE

Article Title: Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio

doi: 10.1371/journal.pone.0189617

Figure Lengend Snippet: The ratio of Th17/Treg in CD4+ T cells in IL-35 group or control group on day 1, 3, 5 after co-culturing. A remarkable difference in the ratio of Th17/Treg in CD4+ T cells was observed under these conditions, particularly on day 5 after co-culture (1.4% in control group vs. 0.1% in IL-35 group, P<0.01). Results are expressed as means ± SEM, from two experiments (n = 3 times/group/experiment). Unpaired t-tests were performed for comparisons between control- and IL-35- groups on corresponding days. *, ** and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: CD4+, CD4+CD25+, IL-17A+, and CD4+CD25- T cells were sorted by using the Stemcell magnetic sorter (cat: 18000), the human/mouse CD4 T cell negative selection kit (cat: 19852), the human/mouse Treg positive selection kit (cat: 18782), following the manufacturer’s instructions.

Techniques: Control, Co-Culture Assay

Journal: PLoS ONE

Article Title: Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio

doi: 10.1371/journal.pone.0189617

Figure Lengend Snippet: Representative histograms showing different Th17/Treg ratio (1:0, 1:0.5, 1:1, 1:2) in the co-culture system. With an increase of Treg ratio, on day 5, the proliferation of the CFSE-labeled Th17 cells was suppressed markedly (suppression ratio rising from 7.82% in 1:0 group to 48.2% in 1:2 group); besides, stronger suppression was observed when IL-35 was added, compared to that in the control group (suppression ratio rising from 20.5% in 1:0 group to 76.7% in 1:2 group).

Article Snippet: CD4+, CD4+CD25+, IL-17A+, and CD4+CD25- T cells were sorted by using the Stemcell magnetic sorter (cat: 18000), the human/mouse CD4 T cell negative selection kit (cat: 19852), the human/mouse Treg positive selection kit (cat: 18782), following the manufacturer’s instructions.

Techniques: Co-Culture Assay, Labeling, Control

Journal: PLoS ONE

Article Title: Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio

doi: 10.1371/journal.pone.0189617

Figure Lengend Snippet: The function of co-cultured islet cells in Th17:Treg = 1:0 group and Th17:Treg = 1:2 group in low glucose level (2.5mM) or high glucose level (16.7mM). The results showed that regardless of the glucose level, islet cells in the Th17/Treg = 1:2 group had better insulin secretory function than that in the Th17/Treg = 1:0 group. Results are expressed as means ± SEM, from two experiments (n = 3 times/group/experiment). Unpaired t-tests were performed for comparisons between control- and IL-35- groups on corresponding days. *, ** and *** denote p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: CD4+, CD4+CD25+, IL-17A+, and CD4+CD25- T cells were sorted by using the Stemcell magnetic sorter (cat: 18000), the human/mouse CD4 T cell negative selection kit (cat: 19852), the human/mouse Treg positive selection kit (cat: 18782), following the manufacturer’s instructions.

Techniques: Cell Culture, Control

Journal: PLoS ONE

Article Title: Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio

doi: 10.1371/journal.pone.0189617

Figure Lengend Snippet: Model of IL-35 regulating Th17/Treg differentiation.

Article Snippet: CD4+, CD4+CD25+, IL-17A+, and CD4+CD25- T cells were sorted by using the Stemcell magnetic sorter (cat: 18000), the human/mouse CD4 T cell negative selection kit (cat: 19852), the human/mouse Treg positive selection kit (cat: 18782), following the manufacturer’s instructions.

Techniques: